Journal: Biomolecules and Biomedicine
Article Title: Therapeutic potential of simvastatin in ALS: Enhanced axonal integrity and motor neuron survival through Apoa4 and Alb modulation
doi: 10.17305/bb.2024.11218
Figure Lengend Snippet: Expression of mRNA and proteins. qRT-PCR results presented the expression levels of PI3K (A), AKT (B), C5 (C), PPARγ (D), C4a (E), C3 (F), C1qB (G), and CRP (H) mRNA in different groups; the expression levels of PI3K, p-PI3K, p-AKT, AKT, PPARγ, Apoa4 (I), and C4a, C5, C3, Alb, C1qB, and CRP (J) in different groups were detected. The levels of p-PI3K, p-AKT, PPARγ, Apoa4, C5, and Alb decreased, while C4a, C3, C1qB, and CRP increased in the SOD1G93A + PBS group compared with the WT group; * P < 0.05, ** P < 0.01, vs WT group; # P < 0.05, ## P < 0.01, vs SOD1 G93A + PBS group. qRT-PCR: Quantitative real-time polymerase chain reaction; SOD1: Superoxide dismutase 1; WT: Wilt-type; PPAR: Peroxisome proliferator-activated receptor.
Article Snippet: Primary antibodies: C4a (1:3000, 22233-1-AP, Proteintech, Wuhan, China); C5 (1:600, A8104, Abclonal, Wuhan, China); C3 (1:10,000, 21337-1-AP, Proteintech); PI3K (1:1000, ab302958, Abcam, UK); p-PI3K p85 alpha (Tyr607) (1:1000, AF3241, Affinity, Jiangsu, China); Alb (1:10,000, 16475-1-AP, Proteintech); p-AKT (Ser473) (1:10,000, 66444-1-Ig, Proteintech); AKT (1:5000, 60203-2-Ig, Proteintech); PPARγ (1:5000, 16643-1-AP, Proteintech); APOA4 (1:8000, 17996-1-AP, Proteintech); C1qB (1:1000, A5339, Abclonal, Wuhan, China); CRP (1:2000, 66250-1-Ig, Proteintech); and GAPDH (1:10,000, 60004-1-Ig, Proteintech).
Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction